Biological Imaging Methods Ontology
A structured controlled vocabulary of sample preparation, visualization and imaging methods used in biomedical research.
Tag: FBbi
[14]C [FBbi_00000123]
[[14]C; metabolically incorporated radioisotope]
[3]H [FBbi_00000127]
[[3]H; metabolically incorporated radioisotope]
[32]P [FBbi_00000124]
[[32]P; metabolically incorporated radioisotope]
[32]P labeled DNA probe [FBbi_00000213]
[[32]P labeled DNA probe]
[32]P labeled RNA probe [FBbi_00000220]
[[32]P labeled RNA probe]
[33]P [FBbi_00000125]
[metabolically incorporated radioisotope; [33]P]
[33]P labeled DNA probe [FBbi_00000214]
[[33]P labeled DNA probe]
[33]P labeled RNA probe [FBbi_00000221]
[[33]P labeled RNA probe]
[35]S [FBbi_00000126]
[[35]S; metabolically incorporated radioisotope]
3D-deconvolution [FBbi_00000357]
[3D-deconvolution; linear method]
4\',6-diamidino-2-phenylindole (DAPI) [FBbi_00000056]
[membrane impermeant probe; 4',6-diamidino-2-phenylindole (DAPI)]
5-SFX fluorescein RNA fill [FBbi_00000091]
[5-SFX fluorescein RNA fill; fluor labeled RNA fill]
7-amino-actinomycin D [FBbi_00000424]
[membrane impermeant probe; 7-amino-actinomycin D]
absorption of illumination [FBbi_00000308]
[imaged parameter; absorption of illumination]
AceFP [FBbi_00000499]
[AceFP; Green fluorescent proteins from Anthozoa]
acetone fixed tissue [FBbi_00000004]
[coagulating-fixative fixed tissue; acetone fixed tissue]
acid phosphatase [FBbi_00000158]
[enzyme label; acid phosphatase]
acridine homodimer [FBbi_00000423]
[membrane impermeant probe; acridine homodimer]
acridine orange [FBbi_00000051]
[acridine orange; AO; membrane-permeant probe]
acrolein fixed tissue [FBbi_00000005]
[acrolein fixed tissue; coagulating-fixative fixed tissue]
acrylic painted graphic [FBbi_00000236]
[acrylic painted graphic]
Aequorea victoria red fluorescent protein tag [FBbi_00000083]
[RFP; Aequorea victoria red fluorescent protein tag]
affinity for specific proteins [FBbi_00000459]
[probe for protein; affinity for specific proteins]
Alexa Fluor 350 phalloidin fill [FBbi_00000101]
[Alexa Fluor 350 phalloidin fill]
Alexa Fluor 488 [FBbi_00000440]
[fluorescent label; Alexa Fluor 488]
Alexa Fluor 488 actin fill [FBbi_00000096]
[Alexa Fluor 488 actin fill]
Alexa Fluor 488 phalloidin fill [FBbi_00000102]
[Alexa Fluor 488 phalloidin fill]
Alexa Fluor 546 [FBbi_00000441]
[Alexa Fluor 546; fluorescent label]
Alexa Fluor 555 [FBbi_00000443]
[fluorescent label; Alexa Fluor 555]
Alexa Fluor 568 [FBbi_00000442]
[fluorescent label; Alexa Fluor 568]
Alexa Fluor 568 actin fill [FBbi_00000097]
[Alexa Fluor 568 actin fill]
Alexa Fluor 594 [FBbi_00000444]
[fluorescent label; Alexa Fluor 594]
Alexa Fluor 610 [FBbi_00000445]
[Alexa Fluor 610; fluorescent label]
Alexa Fluor 633 [FBbi_00000446]
[fluorescent label; Alexa Fluor 633]
Alexa Fluor 647 [FBbi_00000447]
[fluorescent label; Alexa Fluor 647]
alkaline phosphatase [FBbi_00000132]
[enzyme label; alkaline phosphatase]
alpha-bungarotoxin [FBbi_00000464]
[non-immunological protein probe; alpha-bungarotoxin]
AmCyanFP [FBbi_00000487]
[AmCyanFP; Cyan fluorescent proteins from Anthozoa]
aminomethylcoumarin [FBbi_00000191]
[AMCA; aminomethylcoumarin; fluorescent label]
anaxial illumination [FBbi_00000291]
Illumination is distributed asymmetrically around the optic axis, typically achieved by a non-circular stop placed below the lower lens element and aperture of the condenser.
animation [FBbi_00000267]
A form of graphical illustration that changes with time to give a sense of motion or represent dynamic changes in the portrayal.
ANSOM [FBbi_00000349]
Aperture-less NSOM.
antibody [FBbi_00000401]
[antibody; macromolecular probe]
AQ143 [FBbi_00000491]
[Far-red fluorescent proteins from Anthozoa; AQ143]
Arachis hypogaea (PNA) [FBbi_00000428]
[lectin; Arachis hypogaea (PNA)]
area detector [FBbi_00000395]
[area detector; detection method]
array scanning [FBbi_00000289]
Multiple points of illumination generated by a fixed array of sources.
array-scan confocal microscopy [FBbi_00000393]
Fixed array of sources focused to an array of spots that is swept over the specimen in raster fashion
AsRed2FP [FBbi_00000520]
[AsRed2FP; Red fluorescent proteins from Anthozoa]
atomic force microscopy [FBbi_00000259]
the scanning probe is maintained at a fixed distance above the surface e of the specimen by van der Waals forces
aurothioglucose [FBbi_00000576]
[negative staining; aurothioglucose]
avalanche photodiode (APD) [FBbi_00000297]
[spot detector; avalanche photodiode (APD)]
Azami GreenFP [FBbi_00000500]
[Green fluorescent proteins from Anthozoa; Azami GreenFP]
AzuriteFP [FBbi_00000461]
[AzuriteFP; Blue fluorescent protein from Aequorea victoria]
back-scattered_electron imaging [FBbi_00000373]
imaged formed using electrons undergoing elastic scattering at very high angles, with emission of characteristic X-rays
batrachatoxin [FBbi_00000467]
[affinity for specific proteins; batrachatoxin]
beta-galactosidase [FBbi_00000077]
[lacZ, X-gal; beta-galactosidase; genetically encoded enzyme]
beta-glucuronidase [FBbi_00000078]
[GUS, X-gluc; beta-glucuronidase; genetically encoded enzyme]
biotin [FBbi_00000458]
[fluorescent label; biotin]
biotin ethylenediamine fill [FBbi_00000119]
[miscellaneous fill; biotin ethylenediamine fill; Neurobiotin]
biotin labeled DNA probe [FBbi_00000205]
[biotin labeled DNA probe]
biotin labeled RNA probe [FBbi_00000216]
[biotin labeled RNA probe]
black and white graphic [FBbi_00000225]
[black and white graphic; computer graphic]
blind deconvolution [FBbi_00000362]
[3D-deconvolution; blind deconvolution]
Blue fluorescent protein from Aequorea victoria [FBbi_00000435]
[Blue fluorescent protein from Aequorea victoria; fluorescent protein derived from Aequorea victoria]
Blue fluorescent proteins from Anthozoa [FBbi_00000479]
[fluorescent protein derived from Anthozoa; Blue fluorescent proteins from Anthozoa]
BODIPY TR-14-dUTP labeled DNA probe [FBbi_00000208]
[BODIPY TR-14-dUTP labeled DNA probe]
botulinum toxin [FBbi_00000465]
[botulinum toxin; non-immunological protein probe]
boundaries between regions with different refractive index [FBbi_00000599]
[boundaries between regions with different refractive index; source of contrast]
bright-field microscopy [FBbi_00000243]
[wide-field detection; absorption of illumination; bright-field microscopy; makes_use_of; widefield illumination; light microscopy]
bromodeoxyuridine labeled DNA probe [FBbi_00000209]
[bromodeoxyuridine labeled DNA probe]
bsDronpa [FBbi_00000557]
[bsDronpa; photoswitchable fluorescent protein]
c-MYC peptide tag [FBbi_00000086]
[c-MYC peptide tag; small genetically encoded tag]
camera lucida assisted graphic [FBbi_00000228]
[graphic illustration; camera lucida assisted graphic]
CARS [FBbi_00000370]
coherent Raman anti-Stokes microscopy
Cascade blue [FBbi_00000192]
[fluorescent label; Cascade blue]
cationic colloidal gold [FBbi_00000053]
[cationic colloidal gold; optically-dense stain]
CeruleanFP [FBbi_00000468]
[Cyan fluorescent protein from Aequorea; CeruleanFP]
charcoal pencil graphic [FBbi_00000231]
[charcoal pencil graphic; line art graphic]
charge coupled device (CCD) [FBbi_00000294]
[area detector; charge coupled device (CCD)]
chemical composition [FBbi_00000319]
[imaged parameter; chemical composition]
chemically fixed tissue [FBbi_00000002]
[chemically fixed tissue; fixation method]
cholera toxin B [FBbi_00000431]
[cholera toxin B; lectin]
CitrineFP [FBbi_00000474]
[CitrineFP; Yellow fluorescent protein from Aequorea]
CMX rosamine (Mitotracker Red) [FBbi_00000054]
[Mitotracker X; probe for mitochondria; CMX rosamine (Mitotracker Red)]
coagulating-fixative fixed tissue [FBbi_00000003]
[chemically fixed tissue; coagulating-fixative fixed tissue; solvent permeabilized]
cobalt fill [FBbi_00000120]
[miscellaneous fill; cobalt fill]
coherent illumination [FBbi_00000279]
[coherent illumination; illumination method]
color graphic [FBbi_00000226]
[color graphic; computer graphic]
colored pencil graphic [FBbi_00000232]
[colored pencil graphic; line art graphic]
compartmentalization of stain or label [FBbi_00000604]
[source of contrast; compartmentalization of stain or label]
complementary metal oxide semiconductor (CMOS) [FBbi_00000304]
[area detector; complementary metal oxide semiconductor (CMOS)]
computational method [FBbi_00000323]
[computational method; contrast-enhancing method]
computed tomography [FBbi_00001005]
Tomography in which virtual sections are taken from multiple angles and the results are processed via tomographic reconstruction software to produce 2 or 3D images.
computer graphic [FBbi_00000224]
[graphic illustration; computer graphic]
conconavalin A [FBbi_00000425]
[lectin; conconavalin A]
confocal microscopy [FBbi_00000251]
[confocal microscopy; fluorescence emission; narrow-field detection; makes_use_of; narrowfield illumination; fluorescence microscopy]
constrained iterative deconvolution [FBbi_00000360]
[3D-deconvolution; constrained iterative deconvolution]
continuous illumination [FBbi_00000283]
[illumination method; continuous illumination]
contrast-enhancing method [FBbi_00000271]
[contrast-enhancing method; method involved in biological imaging]
CopGFP [FBbi_00000501]
[Green fluorescent proteins from Anthozoa; CopGFP]
copper salt [FBbi_00000610]
[electron-dense stain; copper salt]
cresyl fast violet [FBbi_00000035]
[cresyl fast violet; optically-dense stain]
critical_point dried specimen [FBbi_00000581]
specimen from which water has been removed by a process that avoids liquid-gas and solid-gas phase transitions.
crosslinking-fixative fixed tissue [FBbi_00000008]
[chemically fixed tissue; crosslinking-fixative fixed tissue]
cryofixed tissue [FBbi_00000013]
[cryofixed tissue; fixation method]
cryostat-sectioned tissue [FBbi_00000027]
[cryostat-sectioned tissue]
Cy2 [FBbi_00000448]
[fluorescent label; Cy2]
Cy3 [FBbi_00000449]
[Cy3; fluorescent label]
Cy5 [FBbi_00000450]
[fluorescent label; Cy5]
Cyan fluorescent protein from Aequorea [FBbi_00000436]
[Cyan fluorescent protein from Aequorea; fluorescent protein derived from Aequorea victoria]
Cyan fluorescent proteins from Anthozoa [FBbi_00000480]
[fluorescent protein derived from Anthozoa; Cyan fluorescent proteins from Anthozoa]
CyPet [FBbi_00000469]
[CyPet; Cyan fluorescent protein from Aequorea]
dark-field microscopy [FBbi_00000244]
imaging with rejection of the unscattered illumination (removal of the zero order component of the diffracted wave)
darkfield illumination [FBbi_00000292]
illumination restricted to angles that exceed the acceptance angle of the image-forming lens
defocus phase contrast [FBbi_00000325]
[optical method; defocus phase contrast]
Dendra2FP [FBbi_00000549]
[photoconvertible fluorescent protein; Dendra2FP]
dEOSFP [FBbi_00000550]
[photoconvertible fluorescent protein; dEOSFP]
detection method [FBbi_00000269]
media and devices employed for recording the alterations in the illumination that result from its interaction with the sample
detection of acoustic waves [FBbi_00000376]
[detection of acoustic waves; detection method]
detection of current induced in the specimen [FBbi_00000377]
[detection method; detection of current induced in the specimen]
detection of electrons [FBbi_00000375]
[detection of electrons; detection method]
detection of photons [FBbi_00000374]
[detection of photons; detection method]
detection of UV/visible/IR photons [FBbi_00000378]
[detection of UV/visible/IR photons; detection of photons]
detection of X-ray photons [FBbi_00000379]
[detection of X-ray photons; detection of photons]
detergent permeabilized [FBbi_00000262]
Membrane permeabilization by the action of ionic or non-ionic detergents
DiA [FBbi_00000113]
[DiA; probe for lipid]
diagram [FBbi_00000033]
[diagram; portrayed image]
DiD [FBbi_00000114]
[probe for lipid; DiD]
differences in adsorption or binding of stain [FBbi_00000598]
[source of contrast; differences in adsorption or binding of stain]
differences in amount of elastic light scattering [FBbi_00000600]
[differences in amount of elastic light scattering; source of contrast]
differences in chemical composition [FBbi_00000591]
[source of contrast; differences in chemical composition]
differences in deposition of metal shadow [FBbi_00000601]
[source of contrast; differences in deposition of metal shadow]
differences in fluorescence lifetime [FBbi_00000605]
[differences in fluorescence lifetime; source of contrast]
differences in intrinsic optical density [FBbi_00000602]
[source of contrast; differences in intrinsic optical density]
differences in orientation of fluorophore [FBbi_00000606]
[source of contrast; differences in orientation of fluorophore]
differential interference contrast microscopy [FBbi_00000245]
[polarized light illumination; polarization-sensitive detection; differential interference contrast microscopy; optical path length gradient; makes_use_of; Nomarski; DIC; Wollaston prism; light microscopy]
digoxigenin labeled DNA probe [FBbi_00000206]
[digoxigenin labeled DNA probe]
digoxigenin labeled RNA probe [FBbi_00000217]
[digoxigenin labeled RNA probe]
DiI [FBbi_00000115]
[DiI; probe for lipid]
DiIC16 [FBbi_00000438]
[DiIC16; probe for lipid]
DiO [FBbi_00000116]
[DiO; probe for lipid]
DiOC6 (3,3'-dihexyloxacarbocyanine iodide) [FBbi_00000055]
[DiOC6 (3,3’-dihexyloxacarbocyanine iodide); probe for lipid]
DiR [FBbi_00000117]
[probe for lipid; DiR]
dispersed cells in vitro [FBbi_00000611]
[sample preparation method; dispersed cells in vitro]
distribution of a specific nucleic acid sequence [FBbi_00000596]
[source of contrast; distribution of a specific nucleic acid sequence]
distribution of a specific protein [FBbi_00000597]
[source of contrast; distribution of a specific protein]
distribution of epitope [FBbi_00000592]
[source of contrast; distribution of epitope]
DNA probe [FBbi_00000204]
[DNA probe; sequence-specific nucleic acid probe]
DOPI (defocussed orientation and position imaging) [FBbi_00000338]
[DOPI (defocussed orientation and position imaging); point-localization method]
Dronpa [FBbi_00000558]
[Dronpa; photoswitchable fluorescent protein]
Dronpa-3 [FBbi_00000559]
[Dronpa-3; photoswitchable fluorescent protein]
DsRed [FBbi_00000085]
[DsRed; Orange fluorescent proteins from Anthozoa]
DsRed-ES FP [FBbi_00000540]
[DsRed-ES FP; fluorescent protein timer]
DsRed-Express2FP [FBbi_00000507]
[DsRed-Express2FP; Orange fluorescent proteins from Anthozoa]
DsRed-ExpressFP [FBbi_00000506]
[DsRed-ExpressFP; Orange fluorescent proteins from Anthozoa]
DsRed-MaxFP [FBbi_00000508]
[DsRed-MaxFP; Orange fluorescent proteins from Anthozoa]
DsRed-monomerFP [FBbi_00000509]
[DsRed-monomerFP; Orange fluorescent proteins from Anthozoa]
DsRed2FP [FBbi_00000510]
[DsRed2FP; Orange fluorescent proteins from Anthozoa]
dTomatoFP [FBbi_00000511]
[Orange fluorescent proteins from Anthozoa; dTomatoFP]
dummy term [FBbi_00000000]
[dummy term]
E2GFP [FBbi_00000560]
[E2GFP; photoswitchable fluorescent protein]
EBFP [FBbi_00000080]
[EBFP; Blue fluorescent protein from Aequorea victoria]
ECFP [FBbi_00000081]
[ECFP; Cyan fluorescent protein from Aequorea]
EDAX imaging [FBbi_00000382]
[electron microscopy; EDAX imaging]
EELS imaging [FBbi_00000381]
[electron microscopy; ESI electron spectroscopic imaging; EFTEM energy filtered TEM; EELS imaging]
EGFP [FBbi_00000082]
[EGFP; Green fluorescent protein from Aequorea]
elastic scattering [FBbi_00000310]
[elastic scattering; imaged parameter]
elastic scattering of electrons [FBbi_00000586]
[elastic scattering of electrons]
elastic scattering of photons [FBbi_00000587]
[elastic scattering of photons]
electron bombardment CCD (EBCCD) [FBbi_00000302]
[electron bombardment CCD (EBCCD); area detector]
electron dense label [FBbi_00000457]
[electron dense label; vizualization of label conjugated to probe]
electron density [FBbi_00000315]
[imaged parameter; electron density]
electron microscopy [FBbi_00000256]
[mode of electron microscopy; illumination by electrons; makes_use_of; electron microscopy; microscopy with lenses]
electron multiplying CCD (EMCCD) [FBbi_00000301]
[electron multiplying CCD (EMCCD); area detector]
electron-dense stain [FBbi_00000568]
[stain with broad specificity; electron-dense stain]
elevation [FBbi_00000320]
[imaged parameter; elevation]
embedded tissue [FBbi_00000015]
[sample preparation method; embedded tissue]
EmeraldFP [FBbi_00000471]
[EmeraldFP; Green fluorescent protein from Aequorea]
emission-depletion illumination [FBbi_00000390]
[emission-depletion illumination; specimen_modifying_illumination]
endogenous substrate, acidotropic-fluor conjugate [FBbi_00000070]
[LysoTracker; endogenous substrate, acidotropic-fluor conjugate]
endogenous substrate, diaminobenzidine [FBbi_00000036]
[endogenous substrate, diaminobenzidine]
endogenous substrate, dye conjugate [FBbi_00000069]
[endogenous substrate, dye conjugate]
endogenous substrate, lectin-fluor conjugate [FBbi_00000071]
[endogenous substrate, lectin-fluor conjugate]
endogenous substrate, lectin-gold conjugate [FBbi_00000072]
[endogenous substrate, lectin-gold conjugate]
endogenous substrate, non-vital dye [FBbi_00000032]
[endogenous substrate, non-vital dye]
endogenous substrate, phalloidin-fluor conjugate [FBbi_00000074]
[endogenous substrate, phalloidin-fluor conjugate]
endogenous substrate, vital dye [FBbi_00000050]
[endogenous substrate, vital dye]
enzyme label [FBbi_00000456]
[enzyme label; vizualization of label conjugated to probe]
eosin [FBbi_00000037]
Staining with an aqueous or alcoholic solution of eosin Y,colors eosinophilic structures in various shades of red, pink and orange. The eosinophilic structures are generally composed of intracellular or extracellular protein. The Lewy bodies and Mallory bodies are examples of eosinophilic structures. Most of the cytoplasm is eosinophilic. Red blood cells are stained intensely red.
eosin phalloidin fill [FBbi_00000103]
[eosin phalloidin fill]
eqFP611 [FBbi_00000521]
[Red fluorescent proteins from Anthozoa; eqFP611]
esterase [FBbi_00000176]
[enzyme label; esterase]
ethanol fixed tissue [FBbi_00000006]
[ethanol fixed tissue; coagulating-fixative fixed tissue]
ethidium bromide [FBbi_00000038]
[membrane impermeant probe; ethidium bromide]
ethylene glycol-bis-succinimidyl succinate fixed tissue [FBbi_00000009]
[EGS; ethylene glycol-bis-succinimidyl succinate fixed tissue; crosslinking-fixative fixed tissue]
evanescent wave microscopy [FBbi_00000617]
[evanescent wave microscopy; total internal reflection fluorescence TIRF; fluorescence microscopy]
evanescent wave scattering [FBbi_00000616]
[FTIR frustrated total internal reflection; dark-field microscopy; evanescent wave scattering]
exhaustive photon reassignment [FBbi_00000363]
[3D-deconvolution; exhaustive photon reassignment]
exogenous label [FBbi_00000075]
[exogenous label]
EYFP [FBbi_00000084]
[Yellow fluorescent protein from Aequorea; EYFP]
Far-red fluorescent proteins from Anthozoa [FBbi_00000484]
[fluorescent protein derived from Anthozoa; Far-red fluorescent proteins from Anthozoa]
farfield illumination [FBbi_00000282]
[farfield illumination; illumination method]
Fast-FT [FBbi_00000541]
[Fast-FT; fluorescent protein timer]
film [FBbi_00000303]
[area detector; film]
FIONA (fluorescence imaging with one nanometer accuracy) [FBbi_00000337]
[FIONA (fluorescence imaging with one nanometer accuracy); point-localization method]
fixation method [FBbi_00001012]
[sample preparation method; fixation method]
FLAG peptide tag [FBbi_00000087]
[FLAG peptide tag; small genetically encoded tag]
FLIM [FBbi_00000368]
[FLIM; fluorescence microscopy]
FLIP [FBbi_00000365]
Fluorescence loss in photobleaching; a method of determining whether two populations of fluorophore are in diffusive communication with one another by observing the decrease in brightness of one population when the other population is subjected to continuous photobleaching.
fluor conjugated actin fill [FBbi_00000095]
[fluor labeled protein fill; fluor conjugated actin fill]
fluor conjugated tubulin fill [FBbi_00000109]
[fluor labeled protein fill; fluor conjugated tubulin fill]
fluor labeled DNA probe [FBbi_00000207]
[fluor labeled DNA probe]
fluor labeled protein fill [FBbi_00000094]
[intracellular filling; fluor labeled protein fill]
fluor labeled RNA fill [FBbi_00000090]
[intracellular filling; fluor labeled RNA fill]
Fluorescein (FITC) [FBbi_00000451]
[fluorescent label; Fluorescein (FITC)]
fluoresceine actin fill [FBbi_00000098]
[fluoresceine actin fill]
fluoresceine phalloidin fill [FBbi_00000104]
[fluoresceine phalloidin fill]
fluoresceine-12-dUTP labeled DNA probe [FBbi_00000210]
[fluoresceine-12-dUTP labeled DNA probe]
fluorescence emission [FBbi_00000316]
[imaged parameter; fluorescence emission]
fluorescence microscopy [FBbi_00000246]
[fluorescence emission; UV/visible/IR illumination; makes_use_of; fluorescence microscopy; light microscopy]
fluorescence polarization (polarization angle resolved fluorescence emission) [FBbi_00000318]
[fluorescence polarization (polarization angle resolved fluorescence emission)]
fluorescence polarization microscopy [FBbi_00000603]
[fluorescence microscopy; fluorescence polarization microscopy]
fluorescent dextran fill [FBbi_00000404]
[intracellular filling; fluorescent dextran fill]
fluorescent label [FBbi_00000455]
[fluorescent label; vizualization of label conjugated to probe]
fluorescent protein derived from Aequorea victoria [FBbi_00000420]
[fluorescent protein tag; fluorescent protein derived from Aequorea victoria]
fluorescent protein derived from Anthozoa [FBbi_00000433]
[fluorescent protein derived from Anthozoa; fluorescent protein tag]
fluorescent protein derived from Arabidopsis [FBbi_00000612]
[fluorescent protein tag; fluorescent protein derived from Arabidopsis]
fluorescent protein tag [FBbi_00000405]
[fluorescent protein tag; genetically encoded tag]
fluorescent protein timer [FBbi_00000535]
[optical highlighter; fluorescent protein timer]
fluoroscein labeled RNA probe [FBbi_00000218]
[fluoroscein labeled RNA probe]
focussed ion beam scanning electron microscopy (FIB-SEM) [FBbi_00050000]
A method of reconstructing 3D structure by combining focussed ion beam milling to remove sucessive layers from a sample block with scanning EM to image the exposed surface between each milling step.
form birefringence [FBbi_00000593]
[source of contrast; form birefringence]
formaldehyde fixed tissue [FBbi_00000010]
[formaldehyde fixed tissue; crosslinking-fixative fixed tissue]
FRAP [FBbi_00000366]
Fluorescence loss in photobleaching; a method of determining the rate and extent of exchange between a localized population of fluorophore and a non-localized diffusible pool, by observing the rate of recovery of brightness of the localized population after photobleaching.
free hand graphic [FBbi_00000229]
[free hand graphic; graphic illustration]
freeze_fracture/freeze_etch [FBbi_00000419]
[cryofixed tissue; freeze_fracture/freeze_etch]
freeze-substituted tissue [FBbi_00000621]
[cryofixed tissue; freeze-substituted tissue]
FRET [FBbi_00000367]
Fluorescence (or Forster) resonance energy transfer; a method of estimating the distance between two fluorophores by measuring the extent of non-radiative energy transfer between the two. Experimentally, this involves selective excitation of one while observing emission from the other.
fuchsin [FBbi_00000039]
[fuchsin; optically-dense stain]
gamma ray illumination [FBbi_00001007]
[x-ray/gamma ray illumination; gamma ray illumination]
genetically encoded biotin tag [FBbi_00000418]
[genetically encoded biotin tag; small genetically encoded tag]
genetically encoded enzyme [FBbi_00000076]
[genetically encoded enzyme; genetically encoded tag]
genetically encoded tag [FBbi_00000079]
[macromolecular probe; genetically encoded tag]
giemsa [FBbi_00000040]
[giemsa; optically-dense stain]
glucose oxidase [FBbi_00000178]
[enzyme label; glucose oxidase]
glutaraldehyde fixed tissue [FBbi_00000011]
[glutaraldehyde fixed tissue; crosslinking-fixative fixed tissue]
glycerol permeabilized [FBbi_00000129]
tissue made permeable by the action of glycerol
gold [FBbi_00000202]
[gold; electron dense label]
graphic illustration [FBbi_00000223]
[portrayed image; graphic illustration]
graphite pencil graphic [FBbi_00000233]
[graphite pencil graphic; line art graphic]
Green fluorescent protein from Aequorea [FBbi_00000437]
[fluorescent protein derived from Aequorea victoria; Green fluorescent protein from Aequorea]
Green fluorescent proteins from Anthozoa [FBbi_00000481]
[fluorescent protein derived from Anthozoa; Green fluorescent proteins from Anthozoa]
grey scale graphic [FBbi_00000227]
[grey scale graphic; computer graphic]
ground state depletion scanning (GSD) [FBbi_00000618]
[resolution-enhancing method; ground state depletion scanning (GSD)]
HA peptide tag [FBbi_00000034]
[small genetically encoded tag; HA peptide tag]
hand-sectioned tissue [FBbi_00000028]
[hand-sectioned tissue]
HcRed-tandemFP [FBbi_00000492]
[Far-red fluorescent proteins from Anthozoa; HcRed-tandemFP]
HcRed1FP [FBbi_00000522]
[HcRed1FP; Red fluorescent proteins from Anthozoa]
heat fixed tissue [FBbi_00000609]
[heat fixed tissue; fixation method]
Helix pomatia agglutinin [FBbi_00000429]
[lectin; Helix pomatia agglutinin]
hematoxylin [FBbi_00000041]
The staining method involves application of hemalum, which is a complex formed from aluminium ions and oxidized haematoxylin. This colors nuclei of cells (and a few other objects, such as keratohyalin granules) blue. The staining of nuclei by hemalum does not require the presence of DNA and is probably due to binding of the dye-metal complex to arginine-rich basic nucleoproteins such as histones. The mechanism is different from that of nuclear staining by basic (cationic) dyes such as thionine or toluidine blue. Staining by basic dyes is prevented by chemical or enzymatic extraction of nucleic acids. Such extractions do not prevent staining of nuclei by hemalum.
high-voltage electron microscopy (HVEM) [FBbi_00000622]
[transmission electron microscopy (TEM); high-voltage electron microscopy (HVEM)]
Hoechst 33258 [FBbi_00000417]
[membrane-permeant probe; Hoechst 33258]
Hoechst 33342 [FBbi_00000052]
[Hoechst 33342; HO342; membrane-permeant probe]
Hoffman modulation [FBbi_00000327]
[modified objective lens; Hoffman modulation]
hollow-cone illumination [FBbi_00000290]
illumination parallel or at small angles to the optical axis is attenuated or blocked by a stop
horseradish peroxidase [FBbi_00000138]
[enzyme label; horseradish peroxidase]
illumination by acoustic waves [FBbi_00000274]
[illumination by acoustic waves; illumination method]
illumination by electrons [FBbi_00000273]
[illumination by electrons; illumination method]
illumination by ions [FBbi_00000276]
[illumination method; illumination by ions]
illumination by neutrons [FBbi_00000275]
[illumination method; illumination by neutrons]
illumination by photons [FBbi_00000272]
[illumination by photons; illumination method]
illumination method [FBbi_00000268]
sources and methods for illumination
imaged parameter [FBbi_00000270]
The physical property of the specimen that leads to the modifications of the illumination that are recorded in the image.
imaging method [FBbi_00000222]
[imaging method; method involved in biological imaging]
in-focus phase contrast [FBbi_00000324]
[modified objective lens; in-focus phase contrast]
incoherent illumination [FBbi_00000280]
[incoherent illumination; illumination method]
inelastic scattering [FBbi_00000309]
[imaged parameter; inelastic scattering]
inelastic scattering of electrons [FBbi_00000588]
[inelastic scattering of electrons]
inelastic scattering of photons (Raman scattering) [FBbi_00000589]
[inelastic scattering of photons (Raman scattering)]
intensified CCD (ICCD) [FBbi_00000300]
[area detector; intensified CCD (ICCD)]
intensified SIT (ISIT) [FBbi_00000299]
[intensified SIT (ISIT); area detector]
interference contrast [FBbi_00000330]
[optical method; interference contrast]
interference reflection contrast (IRM) [FBbi_00000329]
[optical method; interference reflection contrast (IRM)]
intermediate voltage electron microacopy (IVEM) [FBbi_00000623]
[intermediate voltage electron microacopy (IVEM); transmission electron microscopy (TEM)]
intracellular filling [FBbi_00000088]
visualization of the connected regions of a cell by filling with a label
intrinsic birefringence [FBbi_00000594]
[source of contrast; intrinsic birefringence]
intrinsic mass distribution [FBbi_00000607]
[source of contrast; intrinsic mass distribution]
IrisFP [FBbi_00000556]
[photoconvertible/photoswitchable fluorescent protein; IrisFP]
isolated subcellular component [FBbi_00000579]
A fragment of a cell, subcellular organelle, or macromolecular complex.
JRedFP [FBbi_00000523]
[Red fluorescent proteins from Anthozoa; JRedFP]
KaedeFP [FBbi_00000551]
[photoconvertible fluorescent protein; KaedeFP]
KatushkaFP [FBbi_00000493]
[Far-red fluorescent proteins from Anthozoa; KatushkaFP]
KFP1 [FBbi_00000561]
[photoswitchable fluorescent protein; KFP1]
Kusabira OrangeFP [FBbi_00000512]
[Orange fluorescent proteins from Anthozoa; Kusabira OrangeFP]
Kusabira OrangeFP2 [FBbi_00000513]
[Orange fluorescent proteins from Anthozoa; Kusabira OrangeFP2]
labeled nucleic acid probe [FBbi_00000203]
[labeled nucleic acid probe]
labeled primary antibody [FBbi_00000130]
[labeled primary antibody]
lead salt [FBbi_00000570]
[electron-dense stain; lead salt]
lectin [FBbi_00000402]
[lectin; macromolecular probe]
light microscopy [FBbi_00000345]
[mode of light microscopy; UV/visible/IR illumination; makes_use_of; microscopy with lenses; light microscopy]
light-sheet illumination [FBbi_00000364]
illumination in the form of a thin sheet of light directed perpendicular to the optic axis
line art graphic [FBbi_00000230]
[graphic illustration; line art graphic]
linear method [FBbi_00000354]
a computational method that is linear in the mathematical sense, meaning that image intensity is conserved and the data remain suitable for all quantitative analyses
living tissue [FBbi_00000025]
Unfixed fully hydrated tissue, typically employed to monitor dynamic changes in the living state.
lucifer yellow [FBbi_00000057]
[lucifer yellow; miscellaneous fill]
lyophilized specimen [FBbi_00000583]
specimen from which water has been removed by evaporation under vacuum at a temperature below freezing.
macromolecular probe [FBbi_00000410]
[visualization by chemical attribute; macromolecular probe]
macroscopy [FBbi_00000240]
methods for imaging objects large enough to be observed by the unaided eye
mAppleFP [FBbi_00000524]
[Red fluorescent proteins from Anthozoa; mAppleFP]
maximum likelihood deconvolution [FBbi_00000361]
[3D-deconvolution; maximum likelihood deconvolution]
mCherryFP [FBbi_00000525]
[Red fluorescent proteins from Anthozoa; mCherryFP]
mCyFP [FBbi_00000488]
[mCyFP; Cyan fluorescent proteins from Anthozoa]
Medium-FT [FBbi_00000542]
[Medium-FT; fluorescent protein timer]
membrane impermeant probe [FBbi_00000422]
[membrane impermeant probe; probes for nucleic acid]
membrane-permeant probe [FBbi_00000421]
[probes for nucleic acid; membrane-permeant probe]
mEOS2FP [FBbi_00000552]
[photoconvertible fluorescent protein; mEOS2FP]
metabolically incorporated radioisotope [FBbi_00000122]
[visualization by chemical attribute; metabolically incorporated radioisotope]
methanol fixed tissue [FBbi_00000007]
[coagulating-fixative fixed tissue; methanol fixed tissue]
method involved in biological imaging [FBbi_root_00000000]
[method involved in biological imaging]
methyl green [FBbi_00000042]
[membrane impermeant probe; methyl green]
methyl violet [FBbi_00000043]
[methyl violet; optically-dense stain]
methylene blue [FBbi_00000058]
[methylene blue; optically-dense stain]
microscopy [FBbi_00000241]
methods for forming images of objects too small to be observed with the unaided eye
microscopy with lenses [FBbi_00000343]
[microscopy with lenses]
microtome-sectioned tissue [FBbi_00000029]
[microtome-sectioned tissue]
miniSOG [FBbi_00000615]
[phototropin 2; miniSOG]
miscellaneous fill [FBbi_00000118]
[miscellaneous fill; intracellular filling]
mKate2FP [FBbi_00000495]
[mKate2FP; Far-red fluorescent proteins from Anthozoa]
mKateFP [FBbi_00000494]
[mKateFP; Far-red fluorescent proteins from Anthozoa]
mKikGR [FBbi_00000553]
[photoconvertible fluorescent protein; mKikGR]
modified objective lens [FBbi_00000353]
Uses an objective with extra components to manipulate the wave front in the plane of the objective aperture.
molybdenum compound [FBbi_00000574]
[negative staining; molybdenum compound]
montage [FBbi_00000261]
[portrayed image; montage]
mOrange2FP [FBbi_00000515]
[Orange fluorescent proteins from Anthozoa; mOrange2FP]
mOrangeFP [FBbi_00000514]
[Orange fluorescent proteins from Anthozoa; mOrangeFP]
mPlumFP [FBbi_00000496]
[Far-red fluorescent proteins from Anthozoa; mPlumFP]
mRasberryFP [FBbi_00000526]
[Red fluorescent proteins from Anthozoa; mRasberryFP]
mRFP1 [FBbi_00000527]
[Red fluorescent proteins from Anthozoa; mRFP1]
mRubyFP [FBbi_00000528]
[Red fluorescent proteins from Anthozoa; mRubyFP]
mStrawberryFP [FBbi_00000529]
[Red fluorescent proteins from Anthozoa; mStrawberryFP]
mTagBFP [FBbi_00000486]
[mTagBFP; Blue fluorescent proteins from Anthozoa]
mTFP0.7 [FBbi_00000562]
[mTFP0.7; photoswitchable fluorescent protein]
mTFP1 [FBbi_00000489]
[mTFP1; Cyan fluorescent proteins from Anthozoa]
multi-photon microscopy [FBbi_00000255]
[fluorescence emission; pulsed illumination; makes_use_of; narrowfield illumination; multi-photon microscopy; fluorescence microscopy]
multiple point scanning [FBbi_00000287]
illumination by multiple points of light simultaneously
mWasabiFP [FBbi_00000502]
[mWasabiFP; Green fluorescent proteins from Anthozoa]
narrow field light micrograph [FBbi_00000250]
[narrow field light micrograph]
narrow-field detection [FBbi_00000307]
[narrow-field detection; detection method]
narrowfield illumination [FBbi_00000278]
illumination restricted to a small portion of the field of view of the imaging lens; often one or more diffraction limited spots
nearest-neighbors deblurring [FBbi_00000359]
[non-linear method; nearest-neighbors deblurring]
nearfield illumination [FBbi_00000281]
[nearfield illumination; illumination method]
nearfield illumination [FBbi_00000619]
[makes_use_of; evanescent wave microscopy; nearfield illumination; illumination method]
nearfield illumination [FBbi_00000620]
[makes_use_of; nearfield illumination; illumination method; evanescent wave scattering]
nearfield scanning optical microscopy (ANSOM) [FBbi_00000348]
[nearfield scanning optical microscopy (ANSOM); scanning probe microscopy]
negative staining [FBbi_00000399]
visualization by exclusion of a stain in which the specimen is immersed; compare with “positive staining.
Nile blue A [FBbi_00000059]
[Nile blue A; optically-dense stain]
Nile red [FBbi_00000060]
[Nile red; probe for lipid]
nitro blue tetrazolium chloride [FBbi_00000044]
[nitro blue tetrazolium chloride; optically-dense stain]
no-neighbors deblurring [FBbi_00000358]
[non-linear method; no-neighbors deblurring]
non-immunological protein probe [FBbi_00000463]
[non-immunological protein probe; macromolecular probe]
non-linear method [FBbi_00000355]
computational method that are mathematically non-linear; image intensity is not conserved, and the data are in general not suitable for quantitative analyses of pixel values
non-specific protein affinity [FBbi_00000460]
[probe for protein; non-specific protein affinity]
nuclear fast red [FBbi_00000045]
[optically-dense stain; nuclear fast red]
oblique illumination [FBbi_00000285]
[oblique illumination; illumination method]
OCT [FBbi_00000371]
optical coherence tomography; an interferometric method of imaging using back-scattered photons (elastic scattering)
oil painted graphic [FBbi_00000237]
[oil painted graphic]
optical highlighter [FBbi_00000434]
[optical highlighter; fluorescent protein tag]
optical method [FBbi_00000322]
[optical method; contrast-enhancing method]
optical path length gradient [FBbi_00000312]
[imaged parameter; optical path length gradient]
optically-dense stain [FBbi_00000567]
[stain with broad specificity; optically-dense stain]
Orange fluorescent proteins from Anthozoa [FBbi_00000482]
[fluorescent protein derived from Anthozoa; Orange fluorescent proteins from Anthozoa]
orcein [FBbi_00000046]
[non-specific protein affinity; orcein]
Oregon Green 488 phalloidin fill [FBbi_00000105]
[Oregon Green 488 phalloidin fill]
Oregon Green 488-5-dUTP labeled DNA probe [FBbi_00000211]
[Oregon Green 488-5-dUTP labeled DNA probe]
Oregon Green 514 phalloidin fill [FBbi_00000106]
[Oregon Green 514 phalloidin fill]
Oregon Green 514 tubulin fill [FBbi_00000110]
[Oregon Green 514 tubulin fill]
organelle-specific probe [FBbi_00000409]
[small-molecule probe; organelle-specific probe]
orientation-independent polarization microscopy [FBbi_00000351]
[orientation-independent polarization microscopy]
osmium tetroxide [FBbi_00000571]
[osmium tetroxide; electron-dense stain]
osmium tetroxide fixed tissue [FBbi_00000012]
[osmium tetroxide fixed tissue; crosslinking-fixative fixed tissue]
other negative stain [FBbi_00000577]
[other negative stain; negative staining]
other radioisotope [FBbi_00000578]
[other radioisotope; metabolically incorporated radioisotope]
PA-CFP [FBbi_00000545]
[PA-CFP; photoactivatable fluorescent protein]
PA-GFP [FBbi_00000544]
[PA-GFP; photoactivatable fluorescent protein]
PA-mCherry1FP [FBbi_00000546]
[PA-mCherry1FP; photoactivatable fluorescent protein]
PA-mRFP [FBbi_00000547]
[PA-mRFP; photoactivatable fluorescent protein]
PadronFP [FBbi_00000563]
[PadronFP; photoswitchable fluorescent protein]
PAINT (point accumulation for imaging in nanoscale topography) [FBbi_00000339]
[PAINT (point accumulation for imaging in nanoscale topography); point-localization method]
painted graphic [FBbi_00000235]
[painted graphic; line art graphic]
PALM (photoactivation localization microscopy) [FBbi_00000335]
[makes_use_of; PALM (photoactivation localization microscopy); point-localization method; photo-activation illumination]
pastel painted graphic [FBbi_00000238]
[pastel painted graphic]
pen and ink graphic [FBbi_00000234]
[black and white graphic; pen and ink graphic; line art graphic]
peptide-nucleic acid probe [FBbi_00000432]
[peptide-nucleic acid probe; sequence-specific nucleic acid probe]
permeabilized tissue [FBbi_00000093]
Tissue treated to make cell membranes permeable, typically for the purpose of allowing access by exogenous stains or substrates.
phalloidin [FBbi_00000100]
[affinity for specific proteins; phalloidin]
Phamret [FBbi_00000548]
[Phamret; photoactivatable fluorescent protein]
phase contrast microscopy [FBbi_00000247]
[phase contrast microscopy; makes_use_of; refractive index; light microscopy; hollow-cone illumination]
Phaseolus vulgaris lectin PHA-L) [FBbi_00000427]
[Phaseolus vulgaris lectin PHA-L)]
PhiYFP [FBbi_00000531]
[Yellow fluorescent proteins from Anthozoa; PhiYFP]
photo-ablation illumination [FBbi_00000385]
[specimen_modifying_illumination; photo-ablation illumination]
photo-activation illumination [FBbi_00000387]
[specimen_modifying_illumination; photo-activation illumination]
photo-bleaching illumination [FBbi_00000384]
[specimen_modifying_illumination; photo-bleaching illumination]
photo-switching illumination [FBbi_00000386]
[specimen_modifying_illumination; photo-switching illumination]
photoactivatable fluorescent protein [FBbi_00000536]
[optical highlighter; photoactivatable fluorescent protein]
photoconvertible fluorescent protein [FBbi_00000537]
[photoconvertible fluorescent protein; optical highlighter]
photoconvertible/photoswitchable fluorescent protein [FBbi_00000538]
[optical highlighter; photoconvertible/photoswitchable fluorescent protein]
photodiode [FBbi_00000296]
[photodiode; spot detector]
photomultiplier tube (PMT) [FBbi_00000295]
[photomultiplier tube (PMT); spot detector]
photoswitchable fluorescent protein [FBbi_00000539]
[optical highlighter; photoswitchable fluorescent protein]
phototropin 2 [FBbi_00000614]
[fluorescent protein derived from Arabidopsis; phototropin 2]
piezo-electric device [FBbi_00000305]
[piezo-electric device; spot detector]
point-localization method [FBbi_00000333]
[resolution-enhancing method; point-localization method]
polarization contrast [FBbi_00000331]
[optical method; polarization contrast]
polarization microscopy [FBbi_00000248]
[retardance; polarized light illumination; polarization-sensitive detection; makes_use_of; polarization microscopy; light microscopy]
polarization-sensitive detection [FBbi_00000352]
[detection method; polarization-sensitive detection]
polarized light illumination [FBbi_00000350]
[illumination by photons; polarized light illumination]
portrayed image [FBbi_00000266]
methods and devices used for creating images by some form of artwork, as distinct from capturing images by means of some recording device
potassium permanganate [FBbi_00000572]
[potassium permanganate; electron-dense stain]
primary antibody plus labeled secondary antibody [FBbi_00000156]
[primary antibody plus labeled secondary antibody]
primary antibody-alkaline phosphastase conjugate, DAB [FBbi_00000133]
[primary antibody-alkaline phosphastase conjugate, DAB]
primary antibody-alkaline phosphastase conjugate, ELF 97 phosphate [FBbi_00000134]
[primary antibody-alkaline phosphastase conjugate, ELF 97 phosphate]
primary antibody-alkaline phosphastase conjugate, Vector Black [FBbi_00000135]
[primary antibody-alkaline phosphastase conjugate, Vector Black]
primary antibody-alkaline phosphastase conjugate, Vector Blue [FBbi_00000136]
[primary antibody-alkaline phosphastase conjugate, Vector Blue]
primary antibody-alkaline phosphastase conjugate, Vector Red [FBbi_00000137]
[primary antibody-alkaline phosphastase conjugate, Vector Red]
primary antibody-aminomethylcoumarin acetate [FBbi_00000146]
[AMCA; primary antibody-aminomethylcoumarin acetate]
primary antibody-Cascade blue [FBbi_00000147]
[primary antibody-Cascade blue]
primary antibody-cyanine [FBbi_00000148]
[Cy2; primary antibody-cyanine]
primary antibody-enzyme conjugate, substrate [FBbi_00000131]
[primary antibody-enzyme conjugate, substrate]
primary antibody-fluor conjugate [FBbi_00000145]
[primary antibody-fluor conjugate]
primary antibody-fluorescein isothiocyanate [FBbi_00000149]
[FITC; primary antibody-fluorescein isothiocyanate]
primary antibody-gold conjugate [FBbi_00000155]
[primary antibody-gold conjugate]
primary antibody-horseradish peroxidase, 3-amino-9-ethylcarbazole [FBbi_00000140]
[primary antibody-horseradish peroxidase, 3-amino-9-ethylcarbazole]
primary antibody-horseradish peroxidase, 3,3',5,5'-tetramethylbenzidine [FBbi_00000141]
[primary antibody-horseradish peroxidase, 3,3’,5,5’-tetramethylbenzidine]
primary antibody-horseradish peroxidase, 4-chloro-1-naphthol [FBbi_00000139]
[primary antibody-horseradish peroxidase, 4-chloro-1-naphthol]
primary antibody-horseradish peroxidase, Vector NovaRed [FBbi_00000142]
[primary antibody-horseradish peroxidase, Vector NovaRed]
primary antibody-horseradish peroxidase, Vector SG [FBbi_00000143]
[primary antibody-horseradish peroxidase, Vector SG]
primary antibody-horseradish peroxidase, Vector VIP [FBbi_00000144]
[primary antibody-horseradish peroxidase, Vector VIP]
primary antibody-indocarbocyanine [FBbi_00000150]
[primary antibody-indocarbocyanine; Cy3]
primary antibody-indodicarbocyanine [FBbi_00000151]
[primary antibody-indodicarbocyanine; Cy5]
primary antibody-rhodamine red-X [FBbi_00000152]
[RRX; primary antibody-rhodamine red-X]
primary antibody-tetramethyl rhodamine isothiocyanate [FBbi_00000153]
[TRITC; primary antibody-tetramethyl rhodamine isothiocyanate]
primary antibody-Texas Red [FBbi_00000154]
[primary antibody-Texas Red; TR]
probe for endoplasmic reticulum [FBbi_00000412]
[probe for endoplasmic reticulum; organelle-specific probe]
probe for Golgi [FBbi_00000413]
[probe for Golgi; organelle-specific probe]
probe for lipid [FBbi_00000112]
[small-molecule probe; probe for lipid]
probe for lysosomes [FBbi_00000414]
[probe for lysosomes; organelle-specific probe]
probe for mitochondria [FBbi_00000411]
[probe for mitochondria; organelle-specific probe]
probe for protein [FBbi_00000408]
[probe for protein; small-molecule probe]
probes for nucleic acid [FBbi_00000406]
[small-molecule probe; probes for nucleic acid]
propidium iodide [FBbi_00000047]
[membrane impermeant probe; PI; propidium iodide]
pulsed illumination [FBbi_00000284]
illumination that varies with time; often achieved with pulsed lasers for the purpose of generating very-high peak illumination power
pyronine [FBbi_00000061]
[pyronine; membrane-permeant probe]
radiography [FBbi_00001000]
An imaging technique that uses high energy electromagnetic radiation, typically X-rays, as a source of illumination.
radioisotope labeled DNA probe [FBbi_00000212]
[radioisotope labeled DNA probe]
radioisotope labeled RNA probe [FBbi_00000219]
[radioisotope labeled RNA probe]
recorded image [FBbi_00000265]
methods and devices used for capturing an image of a real object; distinct from images created by artwork
Red fluorescent proteins from Anthozoa [FBbi_00000483]
[Red fluorescent proteins from Anthozoa; fluorescent protein derived from Anthozoa]
refractive index [FBbi_00000311]
[imaged parameter; refractive index]
resolution-enhancing method [FBbi_00000321]
methods for increasing the resolution beyond the classical Abbe diffraction limit
retardance [FBbi_00000313]
[imaged parameter; retardance]
Rheinberg illumination [FBbi_00000293]
illumination at angles less than the acceptance angle of the image-forming lens is attenuated by a filter to give a colored background of unscattered light; higher angle illumination may be filtered to give a contrasting color
Rhodamine [FBbi_00000452]
[Rhodamine; fluorescent label]
rhodamine 123 [FBbi_00000062]
[rhodamine 123; probe for mitochondria]
rhodamine actin fill [FBbi_00000099]
[rhodamine actin fill]
rhodamine B [FBbi_00000063]
[rhodamine B; optically-dense stain]
rhodamine phalloidin fill [FBbi_00000107]
[rhodamine phalloidin fill]
RNA probe [FBbi_00000215]
[sequence-specific nucleic acid probe; RNA probe]
rsCherryFP [FBbi_00000564]
[rsCherryFP; photoswitchable fluorescent protein]
rsCherryRevFP [FBbi_00000565]
[photoswitchable fluorescent protein; rsCherryRevFP]
rsFastLimeFP [FBbi_00000566]
[rsFastLimeFP; photoswitchable fluorescent protein]
sample preparation method [FBbi_00000001]
[sample preparation method; method involved in biological imaging]
saponin permeabilized [FBbi_00000264]
Saponin, a detergent-like molecule, preferentially forms holes in membranes that contain cholesterol.
saturated structured-illumination microscopy (SSIM) [FBbi_00000340]
structured illumination used at sufficiently high power to cause significant ground-state depletion of the imaged fluorophore, thus allowing for non-linear resolution enhancement
scanning electron microscopy (SEM) [FBbi_00000257]
image is formed by scanning the surface of the specimen with a beam of electrons in a raster pattern
scanning probe microscopy [FBbi_00000344]
imaging by means of a physical probe that passes over the sample
scanning tunneling microscopy [FBbi_00000347]
[scanning tunneling microscopy; scanning probe microscopy]
scanning-transmission electron microscopy [FBbi_00000380]
[scanning-transmission electron microscopy]
SCFP [FBbi_00000470]
[Cyan fluorescent protein from Aequorea; SCFP]
secondary antibody-Alexa Fluor conjugate [FBbi_00000190]
[secondary antibody-Alexa Fluor conjugate]
secondary antibody-alkaline phosphastase conjugate, BCIP [FBbi_00000173]
[secondary antibody-alkaline phosphastase conjugate, BCIP]
secondary antibody-alkaline phosphastase conjugate, DAB [FBbi_00000159]
[secondary antibody-alkaline phosphastase conjugate, DAB]
secondary antibody-alkaline phosphastase conjugate, ELF 97 phosphate [FBbi_00000160]
[secondary antibody-alkaline phosphastase conjugate, ELF 97 phosphate]
secondary antibody-alkaline phosphastase conjugate, Fast Blue B [FBbi_00000161]
[secondary antibody-alkaline phosphastase conjugate, Fast Blue B]
secondary antibody-alkaline phosphastase conjugate, Fast Red [FBbi_00000162]
[secondary antibody-alkaline phosphastase conjugate, Fast Red]
secondary antibody-alkaline phosphastase conjugate, INT [FBbi_00000163]
[secondary antibody-alkaline phosphastase conjugate, INT]
secondary antibody-alkaline phosphastase conjugate, Magenta Phos [FBbi_00000164]
[secondary antibody-alkaline phosphastase conjugate, Magenta Phos]
secondary antibody-alkaline phosphastase conjugate, NABP [FBbi_00000165]
[secondary antibody-alkaline phosphastase conjugate, NABP]
secondary antibody-alkaline phosphastase conjugate, NAGP [FBbi_00000166]
[secondary antibody-alkaline phosphastase conjugate, NAGP]
secondary antibody-alkaline phosphastase conjugate, NAMP [FBbi_00000167]
[secondary antibody-alkaline phosphastase conjugate, NAMP]
secondary antibody-alkaline phosphastase conjugate, NATP [FBbi_00000168]
[secondary antibody-alkaline phosphastase conjugate, NATP]
secondary antibody-alkaline phosphastase conjugate, NBT [FBbi_00000169]
[secondary antibody-alkaline phosphastase conjugate, NBT]
secondary antibody-alkaline phosphastase conjugate, Vector Black [FBbi_00000170]
[secondary antibody-alkaline phosphastase conjugate, Vector Black]
secondary antibody-alkaline phosphastase conjugate, Vector Blue [FBbi_00000171]
[secondary antibody-alkaline phosphastase conjugate, Vector Blue]
secondary antibody-alkaline phosphastase conjugate, Vector Red [FBbi_00000172]
[secondary antibody-alkaline phosphastase conjugate, Vector Red]
secondary antibody-beta-galactosidase, X-Gal [FBbi_00000174]
[secondary antibody-beta-galactosidase, X-Gal]
secondary antibody-beta-glucuronidase, X-gluc [FBbi_00000175]
[secondary antibody-beta-glucuronidase, X-gluc]
secondary antibody-cyanine conjugate [FBbi_00000193]
[Cy2; secondary antibody-cyanine conjugate]
secondary antibody-diaminotriazinylaminoflorescein conjugate [FBbi_00000194]
[secondary antibody-diaminotriazinylaminoflorescein conjugate; DTAF]
secondary antibody-enzyme conjugate, substrate [FBbi_00000157]
[secondary antibody-enzyme conjugate, substrate]
secondary antibody-esterase, Naphthol-AS-D-chloroacetate [FBbi_00000177]
[secondary antibody-esterase, Naphthol-AS-D-chloroacetate]
secondary antibody-fluor conjugate [FBbi_00000189]
[secondary antibody-fluor conjugate]
secondary antibody-fluorescein isothiocyanate conjugate [FBbi_00000195]
[secondary antibody-fluorescein isothiocyanate conjugate; FITC]
secondary antibody-glucose oxidase, TNBT [FBbi_00000179]
[secondary antibody-glucose oxidase, TNBT]
secondary antibody-horseradish peroxidase, 3-amino-9-ethylcarbazole [FBbi_00000183]
[secondary antibody-horseradish peroxidase, 3-amino-9-ethylcarbazole]
secondary antibody-horseradish peroxidase, 3,3'-diaminobenzidine [FBbi_00000180]
[secondary antibody-horseradish peroxidase, 3,3’-diaminobenzidine]
secondary antibody-horseradish peroxidase, 3,3',5,5'-tetramethylbenzidine [FBbi_00000184]
[secondary antibody-horseradish peroxidase, 3,3’,5,5’-tetramethylbenzidine]
secondary antibody-horseradish peroxidase, 3,3\'-diaminobenzidine silver enhanced [FBbi_00000181]
[secondary antibody-horseradish peroxidase, 3,3'-diaminobenzidine silver enhanced]
secondary antibody-horseradish peroxidase, 4-chloro-1-naphthol [FBbi_00000182]
[secondary antibody-horseradish peroxidase, 4-chloro-1-naphthol]
secondary antibody-horseradish peroxidase, Vector NovaRed [FBbi_00000185]
[secondary antibody-horseradish peroxidase, Vector NovaRed]
secondary antibody-horseradish peroxidase, Vector SG [FBbi_00000186]
[secondary antibody-horseradish peroxidase, Vector SG]
secondary antibody-horseradish peroxidase, Vector VIP [FBbi_00000187]
[secondary antibody-horseradish peroxidase, Vector VIP]
secondary antibody-indocarbocyanine conjugate [FBbi_00000196]
[secondary antibody-indocarbocyanine conjugate; Cy3]
secondary antibody-indodicarbocyanine conjugate [FBbi_00000197]
[Cy5; secondary antibody-indodicarbocyanine conjugate]
secondary antibody-lissamine rhodamine sulfonyl chloride [FBbi_00000198]
[LSRC; secondary antibody-lissamine rhodamine sulfonyl chloride]
secondary antibody-phosphatase, Naphthol-AS-BI-phosphate [FBbi_00000188]
[secondary antibody-phosphatase, Naphthol-AS-BI-phosphate]
secondary antibody-rhodamine red-X conjugate [FBbi_00000199]
[secondary antibody-rhodamine red-X conjugate; RRX]
secondary antibody-tetramethyl rhodamine isothiocyanate conjugate [FBbi_00000200]
[secondary antibody-tetramethyl rhodamine isothiocyanate conjugate; TRITC]
secondary antibody-Texas red conjugate [FBbi_00000201]
[secondary antibody-Texas red conjugate; TR]
secondary electron generation [FBbi_00000580]
electrons generated by inelastic scattering of other radiation, the primary radiation.
secondary_electron imaging [FBbi_00000372]
image created using the electrons generated by ionization of a sample due to inelastic scattering of the primary beam of radiation
sectioned tissue [FBbi_00000026]
[sample preparation method; sectioned tissue]
sequence-specific nucleic acid probe [FBbi_00000400]
[sequence-specific nucleic acid probe; macromolecular probe]
serial block face SEM (SBFSEM) [FBbi_00000585]
A method of reconstructing 3D structure by combining serial sectioning with scanning EM.
shadowing and plating [FBbi_00000398]
visualization by depositing a label on the surface of a specimen uniformly (plating), or from a particular direction so that surface topography becomes visible (shadowing)
silicon intensified target tube (SIT) [FBbi_00000298]
[area detector; silicon intensified target tube (SIT)]
silver-intensified cobalt fill [FBbi_00000121]
[silver-intensified cobalt fill]
single point scanning [FBbi_00000286]
illumination by a single spot of light that is scanned across the field of view
single sideband edge enhancement (SSBE) [FBbi_00000328]
[single sideband edge enhancement (SSBE); modified objective lens]
single-spot confocal microscopy [FBbi_00000252]
[makes_use_of; single-spot confocal microscopy; single point scanning; spot detector]
SiriusFP [FBbi_00000462]
[Blue fluorescent protein from Aequorea victoria; SiriusFP]
slit-scan confocal microscopy [FBbi_00000392]
illumination and detection via a thin slit
slit-scanning illumination [FBbi_00000391]
illumination in the shape of a thin line that is swept over the field of view
Slow-FT [FBbi_00000543]
[fluorescent protein timer; Slow-FT]
small genetically encoded tag [FBbi_00000407]
[small genetically encoded tag]
small-molecule probe [FBbi_00000403]
[small-molecule probe; visualization by chemical attribute]
solvent permeabilized [FBbi_00000263]
permeabilization due to extraction of lipid from membranes by an organic solvent such as acetone or methanol.
source of contrast [FBbi_00000590]
The source of the variation in the imaged parameter across the field of view.
soybean agglutinin [FBbi_00000430]
[soybean agglutinin; lectin]
specimen_modifying_illumination [FBbi_00000383]
Illumination used for the purpose of inducing some observable change in the specimen during the course of an imaging session. This illumination may or may not be the same as the illumination used for imaging. Typically used in conjunction with live-cell imaging
SPIM [FBbi_00000369]
single (or selective) plane illumination.
spinning disk confocal microscopy [FBbi_00000253]
[spinning disk confocal microscopy; charge coupled device (CCD); narrow-field detection; makes_use_of; spinning disk scanning]
spinning disk scanning [FBbi_00000288]
scanning by multiple points of light arising from pinholes in a spinning disk that is illuminated by a widefield source
spot detector [FBbi_00000394]
[spot detector; detection method]
stain with broad specificity [FBbi_00000415]
[small-molecule probe; stain with broad specificity; positive staining]
stiffness [FBbi_00000314]
[stiffness; imaged parameter]
stimulated emission depletion (STED) [FBbi_00000334]
[emission-depletion illumination; resolution-enhancing method; makes_use_of; stimulated emission depletion (STED); narrowfield illumination]
STORM (stochastic optical reconstruction microscopy) [FBbi_00000336]
[makes_use_of; photo-switching illumination; point-localization method; STORM (stochastic optical reconstruction microscopy)]
stress-induced birefringence [FBbi_00000595]
[source of contrast; stress-induced birefringence]
structured illumination microscopy (SIM) [FBbi_00000332]
[resolution-enhancing method; structured illumination microscopy (SIM)]
sub-nanosecond time-resolved fluorescence emission) [FBbi_00000317]
[sub-nanosecond time-resolved fluorescence emission)]
SuperfolderFP [FBbi_00000472]
[SuperfolderFP; Green fluorescent protein from Aequorea]
SYFP [FBbi_00000475]
[Yellow fluorescent protein from Aequorea; SYFP]
SYTO Blue [FBbi_00000064]
[SYTO Blue; membrane-permeant probe]
SYTO Green [FBbi_00000065]
[SYTO Green; membrane-permeant probe]
SYTO Orange [FBbi_00000066]
[SYTO Orange; membrane-permeant probe]
SYTO Red [FBbi_00000067]
[SYTO Red; membrane-permeant probe]
SYTOX Green [FBbi_00000048]
[SYTOX Green; membrane impermeant probe]
T-SapphireFP [FBbi_00000473]
[T-SapphireFP; Green fluorescent protein from Aequorea]
TagCFP [FBbi_00000490]
[TagCFP; Cyan fluorescent proteins from Anthozoa]
TagGFP [FBbi_00000503]
[Green fluorescent proteins from Anthozoa; TagGFP]
TagGFP2 [FBbi_00000504]
[Green fluorescent proteins from Anthozoa; TagGFP2]
TagRFP [FBbi_00000516]
[Orange fluorescent proteins from Anthozoa; TagRFP]
TagRFP-T [FBbi_00000517]
[TagRFP-T; Orange fluorescent proteins from Anthozoa]
TagYFP [FBbi_00000532]
[Yellow fluorescent proteins from Anthozoa; TagYFP]
taxol [FBbi_00000073]
[taxol; affinity for specific proteins]
tdKatuskaFP [FBbi_00000497]
[tdKatuskaFP; Far-red fluorescent proteins from Anthozoa]
tdRFP611 [FBbi_00000530]
[Red fluorescent proteins from Anthozoa; tdRFP611]
tdRFP639 [FBbi_00000498]
[Far-red fluorescent proteins from Anthozoa; tdRFP639]
tdTomatoFP [FBbi_00000518]
[tdTomatoFP; Orange fluorescent proteins from Anthozoa]
tetracysteine tag [FBbi_00000416]
[tetracysteine tag; small genetically encoded tag]
Tetramethyl rhodamine (TRITC) [FBbi_00000454]
fluorophore derivative of rhodamine with ex/em 547/572
tetrodotoxin [FBbi_00000466]
[tetrodotoxin; affinity for specific proteins]
Texas Red-X phalloidin fill [FBbi_00000108]
[Texas Red-X phalloidin fill]
TexasRed [FBbi_00000453]
fluorophore with ex/em 589/615
time lapse microscopy [FBbi_00000249]
[light microscopy; time lapse microscopy]
tissue in acrylic resin embedment [FBbi_00000017]
[tissue in acrylic resin embedment; tissue in plastic embedment]
tissue in epoxy resin embedment [FBbi_00000018]
[tissue in plastic embedment; tissue in epoxy resin embedment]
tissue in paraffin embedment [FBbi_00000020]
[tissue in wax embedment; tissue in paraffin embedment]
tissue in plastic embedment [FBbi_00000016]
[tissue in plastic embedment; embedded tissue]
tissue in polyester wax embedment [FBbi_00000021]
[tissue in polyester wax embedment; tissue in wax embedment]
tissue in polyethylene glycol embedment [FBbi_00000022]
[tissue in polyethylene glycol embedment; tissue in wax embedment]
tissue in vitreous ice embedment [FBbi_00000089]
Fully hydrated tissue rapidly frozen to produce vitrified water, and maintained continuously below the glass transition temperature of water (approximately -137 deg C
tissue in wax embedment [FBbi_00000019]
[tissue in wax embedment; embedded tissue]
toluidine blue [FBbi_00000049]
[toluidine blue; optically-dense stain]
tomography [FBbi_00001004]
An imaging technique that creates virtual sections through the use of any kind of penetrating wave.
TopazFP [FBbi_00000476]
[Yellow fluorescent protein from Aequorea; TopazFP]
transmission electron microscopy (TEM) [FBbi_00000258]
[makes_use_of; transmission electron microscopy (TEM); wide-field detection; widefield illumination]
trapping illumination [FBbi_00000389]
[specimen_modifying_illumination; trapping illumination]
tungsten compound [FBbi_00000575]
[negative staining; tungsten compound]
turboRFP [FBbi_00000519]
[Orange fluorescent proteins from Anthozoa; turboRFP]
TurboYFP [FBbi_00000533]
[Yellow fluorescent proteins from Anthozoa; TurboYFP]
two-photon laser scanning microscopy [FBbi_00000254]
[two-photon laser scanning microscopy; multi-photon microscopy]
uncaging illumination [FBbi_00000388]
[specimen_modifying_illumination; uncaging illumination]
unembedded tissue [FBbi_00000023]
[sample preparation method; unembedded tissue]
unfixed tissue [FBbi_00000014]
[unfixed tissue; fixation method]
unprocessed raw data [FBbi_00000582]
data as acquired, with no alterations that change the pixel values.
uranium compound [FBbi_00000573]
[negative staining; uranium compound]
uranyl salt [FBbi_00000569]
[electron-dense stain; uranyl salt]
UV/visible/IR illumination [FBbi_00000341]
[illumination by photons; UV/visible/IR illumination]
VenusFP [FBbi_00000477]
[Yellow fluorescent protein from Aequorea; VenusFP]
vibratome-sectioned tissue [FBbi_00000030]
[vibratome-sectioned tissue]
vidicon tube camera [FBbi_00000613]
[vidicon tube camera; area detector]
visualization by chemical attribute [FBbi_00000397]
methods of visualization that differentiate regions of the specimen on the basis of their chemical properties
visualization method [FBbi_00000031]
[visualization method; method involved in biological imaging]
visualization of contiguous regions [FBbi_00000396]
methods of visualization that differentiate between different regions or surfaces of the specimen based on contiguity
vizualization of label conjugated to probe [FBbi_00000128]
[visualization method; vizualization of label conjugated to probe]
watercolor painted graphic [FBbi_00000239]
[watercolor painted graphic]
wheat germ agglutinin [FBbi_00000426]
[lectin; wheat germ agglutinin]
whole mounted tissue [FBbi_00000024]
[sample preparation method; whole mounted tissue]
wide field light micrograph [FBbi_00000242]
[wide field light micrograph]
wide-field detection [FBbi_00000306]
[wide-field detection; detection method]
widefield illumination [FBbi_00000277]
illumination that covers the entire field of view of the image-forming lens
Wiener filter [FBbi_00000356]
[Wiener filter; linear method]
Wollaston prism [FBbi_00000326]
a birefringent optical element typically used to split polarized illumination into two sets of parallel but displaced rays
wtEOSFP [FBbi_00000554]
[photoconvertible fluorescent protein; wtEOSFP]
wtKIikGR [FBbi_00000555]
[photoconvertible fluorescent protein; wtKIikGR]
X-ray computed tomography [FBbi_00001002]
[X-ray illumination; X-ray CT; X-ray computed tomography; makes_use_of; CAT scan; CT scan; X-ray tomography; computed axial tomography]
X-ray computed tomography [FBbi_00006638]
[X-ray computed tomography]
X-ray fluorescence [FBbi_00000608]
[fluorescence emission; X-ray fluorescence]
X-ray illumination [FBbi_00000342]
[X-ray illumination; x-ray/gamma ray illumination]
X-ray microscopy [FBbi_00000260]
[X-ray radiography; X-ray microscopy; X-ray illumination; makes_use_of]
X-ray radiography [FBbi_00001001]
Radiography using X-rays
X-ray tomography [FBbi_00001003]
Tomography using X-ray illumination.
x-ray/gamma ray illumination [FBbi_00001006]
[x-ray/gamma ray illumination; illumination by photons]
X-Rhodamine [FBbi_00000584]
fluorophore derivative of rhodamine with ex/em 580/605
X-rhodamine RNA fill [FBbi_00000092]
[fluor labeled RNA fill; X-rhodamine RNA fill]
X-rhodamine tubulin fill [FBbi_00000111]
[X-rhodamine tubulin fill]
Yellow fluorescent protein from Aequorea [FBbi_00000439]
[fluorescent protein derived from Aequorea victoria; Yellow fluorescent protein from Aequorea]
Yellow fluorescent proteins from Anthozoa [FBbi_00000485]
[fluorescent protein derived from Anthozoa; Yellow fluorescent proteins from Anthozoa]
YO-PRO-1 [FBbi_00000068]
[membrane impermeant probe; YO-PRO-1]
yPet [FBbi_00000478]
[yPet; Yellow fluorescent protein from Aequorea]
ZsGreenFP [FBbi_00000505]
[Green fluorescent proteins from Anthozoa; ZsGreenFP]
ZsYellowFP [FBbi_00000534]
[Yellow fluorescent proteins from Anthozoa; ZsYellowFP]