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Janelia FlyLight

The FlyLight Project produces large anatomical data sets and highly characterized collections of GAL4, LexA and Split-GAL4 drivers in order to enable the visualization and precise manipulation of individual cell types in the Drosophila nervous system.

SplitExpression_patterntransgeneFlyLightJaneliaLarvalMCFONeuronBridge

Introduction

FlyLight makes its primary data available via VFB as well as through dedicated websites for expression patterns of Generation 1 GAL4 and LexA lines and for split-GAL4 lines. Generation 1, split hemidriver, and stable split combination lines are available primarily from the Bloomington Stock Center. Stable split lines can also be requested via the split-GAL4 website.

FlyLight Data and Resources

FlyLight data is integrated and indexed on VFB and direct linkouts are provided from the Term Info tabs to the original images. VFB hosts the original larval images here. External links to the original collections of adult images are provided below:

Generation 1 GAL4 expression patterns

Generation 1 MCFO (single neuron) patterns

Split-GAL4 Expression Patterns

Janelia Raw Image Collection

Light and EM neurons can also be searched for on NeuronBridge

Also see FlyLight Imaging Tile Documentation.

Datasets

These are the datasets produced through the FlyLight project or otherwise sourced at Janelia, identified from the FlyLight and Janelia source cross-references carried by their images, from the dataset description, or from the release paper. They fall into three kinds: the Generation 1 GMR enhancer-fragment collection with its MCFO single-neuron derivatives, the per-paper split-GAL4 sets characterised in individual publications, and larval material from the Truman flip-out collection.

DatasetStageContentsRecords in VFB
Gen1MCFOTirian2017AdultMCFO images of VT-GAL4 lines from Tirian et al., 201716,965
Gen1MCFOJenett2012AdultMCFO images of GMR-GAL4 lines from Jenett et al., 201215,861
FlyLightGen1Set2019AdultFlyLight - Gen1 GAL4/LexA collection (exported 2019)13,587
TrumanWood2018L3 larvaTruman Larval Flip-Out Collection12,238
Jenett2012AdultFlyLight - GMR GAL4 collection (Jenett2012)3,469
SplitShuai2023AdultSplit-GAL4 lines from Shuai et al., 20232,819
Aso2014AdultMBONs and split-GAL4 lines that target them (Aso2014)2,685
SplitSterne2021AdultSplit-GAL4 lines from Sterne et al., 20211,490
SplitMeissner2024L3 larvaSplit-GAL4 lines from Meissner et al., 2024864
Namiki2018Adultsplit-GAL4 lines for descending neurons (Namiki2018)390
Wu2016Adultsplit-GAL4 lines for LC VPNs (Wu2016)334
Dolan2019AdultGAL4 Split expression patterns from Dolan et al. 2019308
Wolff2018AdultSplits targetting CX neurons, Wolff2018213
Gen1MCFODionne2018AdultMCFO images of GMR-GAL4 lines from Dionne et al., 2018151
FlyLight2019LateralHorn2019AdultFlyLight split-GAL4 lines for Lateral Horn131
FlyLight2019Namiki2018Adultsplit-GAL4 lines for descending neurons (Namiki2018)128
SplitDavis2020AdultSplit-GAL4 lines from Davis et al., 2020100
Robie2017Adultsplit-GAL4 lines for EB neurons (Robie2017)82
SplitWang2020aAdultSplit-GAL4 lines from Wang et al., 2020a70
SplitSchretter2020AdultSplit-GAL4 lines from Schretter et al., 202061
SplitBogovic2020AdultSplit-GAL4 lines from Bogovic et al., 202052
SplitEhrhardt2023AdultSplit-GAL4 lines from Ehrhardt et al., 202333
FlyLight2019Strother2017AdultSplits targetting the visual motion pathway, Strother201732
AsoRubin2016Adultsplit-GAL4 lines for dopaminergic neurons (AsoRubin2016)30
FlyLight2019Wolff2018AdultSplits targetting CX neurons, Wolff201821
SplitVogt2016AdultSplit-GAL4 lines from Vogt et al., 201621
Strother2017AdultSplits targetting the visual motion pathway, Strother201720
Klapoetke2017AdultSplit-GAL4 lines from Klapoetke et al. 201717
SplitMinegishi2023AdultSplit-GAL4 lines from Minegishi et al., 202316
SplitMontague2019AdultSplit-GAL4 lines from Montague et al., 201915
SplitSitaraman2015AdultSplit-GAL4 lines from Sitaraman et al., 201514
FlyLight2019Wu2016Adultsplit-GAL4 lines for LC VPNs (Wu2016)13
SplitCheong2023AdultSplit-GAL4 lines from Cheong et al., 202310
SplitFeng2020AdultSplit-GAL4 lines from Feng et al., 202010
SplitTurner_Evans2020AdultSplit-GAL4 lines from Turner-Evans et al., 20209
SplitBaker2022AdultSplit-GAL4 lines from Baker et al., 20228
SplitJovanic2019LarvaSplit-GAL4 lines from Jovanic et al., 20198
SplitRubin2024AdultSplit-GAL4 lines from Rubin et al., 20248
SplitMorimoto2020AdultSplit-GAL4 lines from Morimoto et al., 20207
SplitNamiki2022AdultSplit-GAL4 lines from Namiki et al., 20227
Hampel2015AdultGrooming neurons and drivers (Hampel 2015)6
SplitKind2021AdultSplit-GAL4 lines from Kind et al., 20216
SplitIsaacson2023AdultSplit-GAL4 lines from Isaacson et al., 20235
SplitWang2020bAdultSplit-GAL4 lines from Wang et al., 2020b5
Gen1MCFOPfeiffer2010AdultMCFO images of GMR-GAL4 lines from Pfeiffer et al., 20104
SplitGao2019AdultSplit-GAL4 lines from Gao et al., 20194
SplitKlapoetke2022AdultSplit-GAL4 lines from Klapoetke et al., 20224
SplitZhao2023AdultSplit-GAL4 lines from Zhao et al., 20234
SplitGiraldo2018AdultSplit-GAL4 lines from Giraldo et al., 20183
SplitMasek2015AdultSplit-GAL4 lines from Masek et al., 20153
SplitXie2021AdultSplit-GAL4 lines from Xie et al., 20213
SplitYamagata2015AdultSplit-GAL4 lines from Yamagata et al., 20153
SplitLongden2023AdultSplit-GAL4 lines from Longden et al., 20232
SplitSchlichting2019AdultSplit-GAL4 lines from Schlichting et al., 20192
TrumanWood2018publicL3 larvaTruman Larval Flip-Out Collection2
FlyLight2019AsoRubin2016Adultsplit-GAL4 lines for dopaminergic neurons (AsoRubin2016)1
FlyLight2019Klapoetke2017AdultSplit-GAL4 lines from Klapoetke et al. 20171
FlyLight2019Robie2017Adultsplit-GAL4 lines for EB neurons (Robie2017)1
SplitTurner_Evans2017AdultSplit-GAL4 lines from Turner-Evans et al., 20171

Records are the individuals VFB holds from each dataset — whole expression patterns, fragments and single neurons.

Datasets from Janelia laboratories that were deposited directly rather than through the FlyLight project are listed under Contributing laboratories.

VT lines are listed under VDRC, including the subset re-imaged through the FlyLight pipeline.

Citations are on each dataset’s own page on VFB; cite the original study rather than VFB when you use the images.